Dark field illumination stage for agar plates
A illumination stage for agar plates to image bacterial colonies without light reflectionsEducation
Creative Commons Attribution Noncommercial Share Alike
Commercial use is not allowed, you must attribute the creator, you may remix this work and the remixed work should be made available under this license.
John S. Parkinson (2009): http://chemotaxis.biology.utah.edu/Parkinson_Lab/publications/PDFs/Parkinson,%202007b.pdf
This stage is developed as part of a open science hardware project for bacterial swarming motility assessment (MBac).
It is a stand-alone module which can be used to take images of agar plates.
For illumination use a standard LED strip and glue it to the inside of the main body. You also need to cover the bottom of the main body with black velvet (I uses black velvet glue foil).
We will be working on an add-on to add a Raspberry Pi camera to the setup.
Materials and methods
Both parts were printed in PLA. The main body on an Anet A8 and the lid on a Ultimaker 3 with PVA supports.
For the main body use white and for the lid black filament.
Issues are used to track todos, bugs or requests. To get started, you could create an issue.