RLP30 + NLP protein models

Protein models of Receptor like protein 30 from Arabidopsis thaliana and a Necrosis inducing protein


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Protein models of an Arabidopsis thaliana receptor and its ligand.

First, the ectodomain of RLP30, which belongs to the leucine-rich repeat receptor-like proteins (LRR-RLPs) and is required for the perception of the MAMP SCFE1 coming from Sclerotinia sclerotiorum (Zhang et al 2013)

And necrosis and ethylene-inducing peptide 1 (Nep1)-like protein (NLP), which is a widespread toxin present in bacteria, fungi and oomycetes (Ottmann et al, 2009; Lenarčič et al, 2017)

EDIT: Added a a base for the RLP30. I used a 100 mm (diameter 2 mm) metal rod to hold the protein model.

Ottmann C, Luberacki B, Küfner I, et al. A common toxin fold mediates microbial attack and plant defense. Proceedings of the National Academy of Sciences of the United States of America. 2009;106(25):10359-10364. doi:10.1073/pnas.0902362106.

Lenarčič T, Albert I, Böhm H, et al. Eudicot plant-specific sphingolipids determine host selectivity of microbial NLP cytolysins. Science. 2017;358(6369):1431-1434. doi:10.1126/science.aan6874.

Zhang W, Fraiture M, Kolb D, et al. Arabidopsis RECEPTOR-LIKE PROTEIN30 and Receptor-Like Kinase SUPPRESSOR OF BIR1-1/EVERSHED Mediate Innate Immunity to Necrotrophic Fungi. The Plant Cell. 2013;25(10):4227-4241. doi:10.1105/tpc.113.117010.

Materials and methods

RLP30 was printed with an Ultimaker Original + and NLP with an Ultimaker 3.
Both were printed with PLA, RLP30 with standard supports and NLP with PVA supports



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Mini 20026476  1 Alexander Kutschera published this design ago